RNA sequencing analysis showing the genes that are differentially regulated by ZNF528-WT or ZNF528-c.1282C>T

RNA was prepared from Saos-2 cells overexpressing either V5-ZNF528-WT or V5-ZNF528-c.1282C>T and control cells expressing empty vector each with two replicates. Illumina TruSeq Stranded mRNA Library preparation kit (Illumina, San Diego, CA, USA) was used according to the manufacturer's instructions. Illumina NextSeq550 platform was used to sequence the RNA libraries, with single-ended and 76 cycle mode. The FASTQ data was prepared within BaseSpace (Illumina, San Diego, CA, USA).