ChIP-seq of ZEB1 in MCF7-V breast carcinoma cell line transduced with a doxycyline-inducible construct to express ZEB1

The epithelial to mesenchymal transition (EMT) is implicated in the metastatic spread of breast cancer cells. EMT transcription factors (TF) regulate different stages of EMT states. In breast cancers, estrogen receptor a (ERa) maintains the epithelial characteristics of breast tumors and is indispensable for efficient endocrine therapy. In this study we investigate whether and how ZEB1, an EMT-TF affects ERa signaling at early stages of EMT and metastasis. Overall design: We did ZEB1 ChIP-seq of cells stably expressing a doxycyline-inducible construct to express ZEB1 in presence of doxycycline. To induce ZEB1 in stable cell line we used 2 µg/ml doxycyline (DOX).