Flow cytometric discrimination of seven lineage markers by using two fluorochromes

Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex cell populations. We present a staining method that identifies major human mononuclear lymphoid and myeloid populations (CD4+ and CD8+ T cells, gd T cells, B cells, NK cells and monocytes), using only two fluorochromes and a minimal number of cells. Our approach increases the number of markers recordable on most flow cytometers allowing for a deeper and more comprehensive immunophenotyping.
Conclusion:
In conclusion, we present a method to expand the number of parameters recordable on flow cytometers allowing for a more exhaustive study of the repertoire of immune cells that can be characterized in a quick, robust and cost-efficient manner. Similar approaches, aiming at expanding the number of recordable markers, may also be developed for different type of animal models or other applications beyond human immune cell profiling. Unstained and controls done with the same markers in different fluorchromes