Effects of the wMel strain of Wolbachia (MGYP2 line) on CHIKV infection and dissemination in Ae. aegypti 12 days after virus exposure.

Virus infection determined using CC-EIA and TaqMan RT-PCR assays (shown in bold), in mosquitoes exposed to CHIKV using oral feeding (experiments 1 and 2) and intrathoracic inoculations (experiments 3 and 4). The Body category refers to the infection rate in the body remnants that remained after the salivary glands were removed. Wolbachia-uninfected lines = MGYP2.tet and Cairns3. N = total number of mosquitoes tested.*PP). For clarity, we only denote significant differences between MGYP2 and its tetracycline-treated counterpart.