Single cell RNA sequencing reveals functional heterogeneity and sex differences of glioma-associated brain macrophages

Single cell RNA-seq analysis of CD11b+ cells sorted from murine syngeneic gliomas, indicating distinct activity of microglia, infiltrating monocytes/macrophages and CNS border associated macrophages. It demonstrates a molecular heterogeneity in the innate immune response in gliomas. Differently expressed genes were identified in activated microglia from glioma-bearing mice of different sex, and profound overexpression of the MHCII genes was observed in male microglial cells. Overall design: Single cell RNA sequencing analysis of 4 groups: female control (2 samples), female tumor (14 days post implantation, 2 samples), male control (2 samples), male tumor (14 days post implantation, 2 samples).