Transcriptomic analysis of embryonic hearts with conditional deletion of Wt1

We performed a conditional ablation of Wt1 in the myocardium of mice embryos. RNASeq analysis identified differential expression of 137 genes in the E13.5 mutant heart as compared to controls. GO functional enrichment analysis suggested that both calcium ion regulation and modulation of potassium channels are deeply altered in the mutant myocardium mRNA profiles of E13.5 wild type (WT) and TnnT2Cre;Wt1flox mutant embryos我们对小鼠胚胎心肌组织中的Wt1基因实施了条件性敲除。通过RNA测序（RNASeq）分析，相较于对照组，E13.5期突变心脏中共鉴定出137个差异表达基因。基因本体（GO）功能富集分析结果显示，在E13.5期野生型（WT）与TnnT2Cre;Wt1flox突变胚胎的心肌组织mRNA表达谱中，突变组的钙离子调控与钾离子通道调控过程均发生了显著改变。