Eutrema japonicum 'Magic' genome assembly & annotation

Wasabi (<i>Eutrema japonicum</i>) is a plant in the Brassicaceae family that produces its distinctive pungent taste through allyl isothiocyanate. This study achieved a high-quality chromosome-level genome assembly of the <i>E. japonicum</i> 'Magic,' bred in Korea for its rapid growth cycle. The assembly was accomplished using a combination of Illumina, PacBio HIFI, Nanopore MinION, and Pore-C scaffolding technologies. The final assembled genome size is 794.6 Mb, anchored to 14 chromosomes. The genome comprises 65.17% repetitive elements and has a BUSCO score of 99.3%, indicating a high level of completeness. Compared to previously published assemblies, the total length increased by approximately 48.08 Mb, while the number of Ns decreased from 89,000 to 49,000, and the assembly gaps (500N padding) reduced from 178 to 98, resulting in a higher quality assembly. This genome will be a valuable resource for genetic and biological research on <i>E. japonicum</i>, aiding in its breeding and genetic improvement.