Analysis of differential miRNA expression in mineralising DPCs treated with pharmacological epigenetic inhibitors.

To establish a miRNA expression profile for DPCs undergoing epigenetically-mediated mineralisation, rodent DPCs were induced to mineralise and treated with a HDAC inhibitor, SAHA, and a DNMT inhibitor, 5-AZA-CdR. RNA was then isolated from DPCs at day 4 of culture and subjected to RNA sequencing. Subsequent bioinformatic analysis identified differentially expressed miRNAs compared with untreated mineralising DPCs. Identification of differentially expressed miRNAs in mineralising rodent DPCs treated with epigenetic inhibitors compared with untreated mineralising DPCs and with non-mineralising DPCs.