Characteristics of the initial and optimized libraries.

aLib1.0 was constructed using single stranded circles for the RCA amplification.bLib2.0 was constructed using double stranded circles for the RCA amplification.cLib2.1 was constructed from Lib2.0 in two steps. First, in-frame sequences were recovered by filtration of the phage library on an anti-Flag antibody; second, the filtrated library was shuffled using only modules preselected to be in frame. The total number of clones for each library is indicated in the library size column.d, eThe ratio of coding sequences was determined from the sequence of randomly picked clones as the number of correct sequences versus the total number of sequences. “In-frame clones” (e) include all coding sequences while “in-frame clones (n ≥ 1)” (f) include coding sequences with at least one motif between the N-cap and the C-cap.f, gThe average number of motifs was calculated as the mean of inserted motifs in all coding sequences (g) or in all coding sequences with at least one motif between the N-cap and the C-cap (h).