amplicon_16S_RADfastqoutput_05022024.tar

16S and 23S ribosomal RNA gene fragments sequenced by a MinION nanopore system were used to obtain sub-species resolution of datasets from a D6322 mock bacterial microbiome. Correct strain resolution usually resulted over a 3 order of magnitude variation in species proportions.

采用MinION纳米孔测序系统对16S和23S核糖体RNA基因片段进行测序，以获取D6322模拟细菌微生物组数据集的亚种分辨率。通常，在物种比例上超过三个数量级的变异中，能够实现菌株的精确分辨。