10x Single-cell Gene expression analysis of pMHC-NP-induced Tet+ cells and anti-CD3-induced IL-10-producing cells

To further explore the transcriptional homogeneity of Tet+ cells with high numbers, we performed scRNAseq of FACS-sorted BDC2.5mi/IAg7 and InsB13-21/IAg7 Tet+ and Tconv cells from pMHCII-NP-treated animals, using the 10x Genomics platform. To study the effect of Bcl6-KO, we also studied transcriptional differences between WT and Bcl6-KO a-CD3-induced IL-10-producing T cells Overall design: Treat NOD mice with BDC2.5mi/IAg7s or InsB13-21/IAg7-NPs to obtain Tet+ and Tconv T cells and perform scRNAseq with 10x technology and treat NOD or NOD CD4.cre.Bcl6(fl/fl) with a-CD3 antibody to obtain IL-10-producing CD4+ T cells and perform scRNAseq