Data from: Collateral sensitivity interactions between antibiotics depend on local abiotic conditions

Mutations conferring resistance to one antibiotic can increase (cross
resistance) or decrease (collateral sensitivity) resistance to others.
Antibiotic combinations displaying collateral sensitivity could be used in
treatments that slow resistance evolution. However, lab-to-clinic
translation requires understanding whether collateral effects are robust
across different environmental conditions. Here, we isolated and
characterized resistant mutants of Escherichia coli using five
antibiotics, before measuring collateral effects on resistance to other
paired antibiotics. During both isolation and phenotyping, we varied
conditions in ways relevant in nature (pH, temperature, bile). This
revealed local abiotic conditions modified expression of resistance
against both the antibiotic used during isolation and other antibiotics.
Consequently, local conditions influenced collateral sensitivity in two
ways: by favouring different sets of mutants (with different collateral
sensitivities), and by modifying expression of collateral effects for
individual mutants. These results place collateral sensitivity in the
context of environmental variation, with important implications for
translation to real-world applications.