RNA sequencing to study the molecular relationships between temperature, aging, and NPR-8

To gain molecular insights into NPR-8-dependent longevity response to temperature, we employed RNA-seq to compare gene expression in young and old wild-type and npr-8(ok1439) animals propagated at different temperatures. Five replicates of eight groups of RNA samples (0-day-old and 9-day-old adult wild-type and npr-8(ok1439) animals propagated at 20°C and 25°C for at least two generations) were collected and subjected to RNA-seq analysis.