aThe unique repeats of LigB from Leptospira interrogans Copenhageni L1-130 were subcloned in pET-20b(+) at the Nde I/Xho I site, which adds an initiating methionine and an LEH6 tail to the Lig protein
aThe unique repeats of LigB from Leptospira interrogans Copenhageni L1-130 were subcloned in pET-20b(+) at the Nde I/Xho I site, which adds an initiating methionine and an LEH6 tail to the Lig protein
aLigA protein designations list the first and last domains included in the construct; 7′ indicates a half domain.bCoordinates refer to the first and last amino acids in the LigA protein of L. interrog
Enzyme activities in solution were determined as described. Km values are for the substrates, glyceraldehyde 3-phosphate (GAP) for TPI and 3-phosphoglyceric acid (3-PGA) for GAPDHS.
