Dental derived stem cell conditioned media for hair growth stimulation

Alopecia is a clinical condition caused by excessive hair loss which may result in baldness, the causes of which still remain elusive. Conditioned media (CM) from stem cells shows promise in regenerative medicine. Our aim was to evaluate the potential CM of dental pulp stem cells obtained from human deciduous teeth (SHED-CM) to stimulate hair growth under in vitro and in vivo conditions. SHED and hair follicle stem cells (HFSCs) (n = 3) were cultured in media combinations; i) STK2, ii) DMEM-KO+10% FBS, iii) STK2+2% FBS and profiled for the presence of positive hair growth-regulatory paracrine factors; SDF-1, HGF, VEGF-A, PDGF-BB and negative hair growth-regulatory paracrine factors; IL-1α, IL-1β, TGF-β, bFGF, TNF-α, and BDNF. The potential of CM from both cell sources to stimulate hair growth was evaluated based on the paracrine profile and measured dynamics of hair growth under in vitro conditions. The administration of CM media to telogen-staged synchronized 7-week old C3H/HeN female mice was carried out to study the potential of the CM to stimulate hair growth in vivo. SHED and HFSCs cultured in STK2 based media showed a shorter population doubling time, higher viability and better maintenance of MSC characteristics in comparison to cells cultured in DMEM-KO media. STK2 based CM contained only two negative hair growth-regulatory factors; TNF-α, IL-1 while DMEM-KO CM contained all negative hair growth-regulatory factors. The in vitro study confirmed that treatment with STK2 based media CM from passage 3 SHED and HFSCs resulted in a significantly higher number of anagen-staged hair follicles (p

脱发是一种由过度脱发引起的临床疾病，可能导致秃顶，其病因尚无定论。源自干细胞的条件培养基（CM）在再生医学领域展现出巨大潜力。本研究旨在评估从人类乳牙中获取的牙髓干细胞（SHED-CM）的潜在CM在体外和体内条件下刺激毛发生长的能力。SHED和毛囊干细胞（HFSCs）（n = 3）在培养基组合中进行培养；i）STK2，ii）DMEM-KO+10%胎牛血清，iii）STK2+2%胎牛血清，并对其阳性毛发生长调节性旁分泌因子（如SDF-1、HGF、VEGF-A、PDGF-BB）和阴性毛发生长调节性旁分泌因子（如IL-1α、IL-1β、TGF-β、bFGF、TNF-α、和BDNF）的存在进行鉴定。基于旁分泌谱和体外条件下毛发生长的动态测量，评估了两种细胞来源的CM刺激毛发生长的潜力。将CM培养基给予同步的7周龄C3H/HeN雌性小鼠的休止期阶段，以研究CM在体内刺激毛发生长的潜力。与DMEM-KO培养基相比，在STK2培养基中培养的SHED和HFSCs表现出更短的倍增时间、更高的活力和更好的MSC特征维持。基于STK2的CM仅包含两种阴性毛发生长调节因子；TNF-α和IL-1，而DMEM-KO CM则包含所有阴性毛发生长调节因子。体外研究表明，使用来自第3代SHED和HFSCs的STK2培养基CM进行处理，显著增加了生长期毛囊的数量（p