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Interactome of chromatin-associated RNA Polymerase II from U2OS cells

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https://www.omicsdi.org/dataset/pride/PXD054701
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To determine the interactome of RNA Polymerase II (RNAPII), HA-tagged RPB3 was stably expressed in U2OS cells. The cells were harvested followed by the native stepwise isolation of chromatin-associated RNAPII complexes. HA-RPB3 was immunoprecipitated and interacting proteins analyzed by quantitative mass spectrometry. U2OS cells expressing no HA-tagged protein were used for comparison.
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2024-12-20
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