EST-SSR genotyping data from: Ecotype variation in the endemic tree Callicarpa subpubescens on small oceanic islands: Genetic, phenotypic, and environmental insights

Callicarpa subpubescens, endemic to the Ogasawara Islands, is suggested to have multiple ecotypes in the Hahajima Islands, specifically in the central part of the Ogasawara Islands. In this study, associations between genetic groups and spatial distribution, habitat, leaf morphology, size structure, and flowering time of each genetic group were investigated on Hahajima and the satellite Imoutojima Islands. Genetic groups were identified using EST-SSR markers, revealing four ecotypes named based on morphological features: Dwarf (D), Glabrescent (G), Tall (T), and Middle (M), with M being a result of the hybridization of G and T. Ecotype D, adapted to dry environments, is characterized by small tree size, dense thick leaves with abundant hairs, and is distributed in dry scrub. Ecotype G, adapted to understory of mesic forests, lacks leaf hairs. Ecotype T, adapted to the canopy of mesic forests, has hairy leaves and is tall in tree height. Ecotype M, adapted to the canopy of mesic scrub or..., Genotypes of each sample were characterized by the 17 EST-SSR markers, which were developed for Callicarpa subpubescens (Setsuko et al. 2018). PCR was carried out in 6 µl reaction mixtures containing ca. 1 ng genomic DNA, 2.5 µl Type-it Multiplex PCR Master Mix (Qiagen, Hilden, Germany), and 0.2 µM of each primer. PCR conditions were as follows: 95°C for 5 min, then 35 or 38 cycles of 94°C for 30 s, 55°C or 60°C for 90 s, 72°C for 90 s, followed by final extension at 60°C for 30 min. PCR fragments were then separated using a 3130 Genetic Analyzer (Applied Biosystems, CA, USA) and genotyped using GeneMarker software (SoftGenetics, PA, USA).
 , , # EST-SSR genotyping data from: Ecotype variation in the endemic tree Callicarpa subpubescens on small oceanic islands: Genetic, phenotypic, and environmental insights

[https://doi.org/10.5061/dryad.6q573n65w](https://doi.org/10.5061/dryad.6q573n65w)

This study investigated associations between genetic groups and ecological and morphological traits in *Callicarpa subpubescens* on the Hahajima and Imoutojima Islands of the Ogasawara Islands, revealing four ecotypes adapted to different habitats and displaying partial reproductive isolation. This data indicates EST-SSR genotypes of all 716 individuals characterized by the 17 EST-SSR markers, which were developed for *Callicarpa subpubescens* (Setsuko et al. 2018).

## Description of the data and file structure

MS Excel sheet \"SupplementaryData1\" contains 17 EST-SSR genotypes of all studied 716 individuals. Column A contains individual IDs, column B contains the names of the islands to which the individuals belong, columns C through AD ...