Targeted activation of CREB in reactive astrocytes is neuroprotective in focal acute cortical injury.. Mus musculus

Transcriptome profiling of mice overexpressing a constitutively active form of CREB in astrocytes (VP16-CREB) vs WT mice, both in normal conditions and after a focal cryolesion (C) in the parietal cortex. Goal is to determine which astrocytic genes are responsible for the neuroprotection we observed in VP16-CREB mice after injury. Overall design: Four group experiment. Loop design in which each sample was hybridized four times, and balanced with respect to the use of Cy3 and Cy5 in the samples. Biological replicates: WT: 6 replicates; T: 5 replicates; WTC: 5 replicates; TC: 6 replicates