Polθ origin of PolIA by Isogai & Wei et al.

These files were analyzed in Isogai & Wei et al. "Diversity and evolution of DNA polymerase θ in eukaryotes and the origin of mitochondrion-localized DNA polymerase PolIA in Euglenozoa.
264DNAP_alignment_trimmed.fasta: A trimmed alignment comprising 202 Polθ, 33 PolIA, four acPolA, and 12 bacterial PolI sequences.
264DNAP_alignment_NOTtrimmed.fasta: This alignment is identical to 264DNAP_alignment_trimmed, but the positions ambiguously aligned and/or those that contain a large number of gaps remain.
264DNAP_ML.treefile: The maximum likelihood (ML) tree with SH-aLRT and UFBoot2 support values inferred from 264DNAP_alignment_trimmed.fasta. Newick formatted.
Kinetoplastea_phylogenomic_31otu325proteins.fas: A phylogenomic alignment comprising 325 proteins. This was used to infer the relationship among 21 kinetoplastids, three diplonemids, and three euglenids. See Table S2 for the details.
Kinetoplastea_phylogenomic_31otu325proteins.treefile: The ML tree inferred from Kinetoplastea_phylogenomic_31otu325proteins.fas. Support values from UFBoot2 and nonparametric bootstrap are included. Newick formatted.
Testtrees_AUtest_Fig3.treefile: The test trees used in the AU test that examined the position of the PolIA clade among Polθ sequences. The corresponding results are shown in Fig. 3. Newick formatted.
Testtrees_AUtest_FigS1.treefile: The test trees used in the AU test that examined the position of two distinct clades of euglenozoan Polθ sequences (i.e., Euglenozoan clade-1 and clade-2). The corresponding results are shown in Fig. S1. Newick formatted.
Testtrees_AUtest_FigS2.treefile: The test trees used in the AU test that examined the position of the clade of the Polθ sequences of two diplomonads, Hemistasia and Namystynia. The corresponding results are shown in Fig. S2. Newick formatted.
Procryptobia_Trinity.fasta: An assembly file of Mi-Seq reads of the kinetoplastid Procryptobia sorokini.
Eug1_over500.fasta.json: The results of domain structure prediction on 22 eugPolθ-1 sequences using InterProScan.
Eug2_over500.fasta.json: The results of domain structure prediction on 18 eugPolθ-2 sequences using InterProScan.
Poltheta_others_over500.fasta.json: The results of domain structure prediction on 106 Polθ sequences using InterProScan.
PolIA_over500.fasta.json: The results of domain structure prediction on 26 PolIA sequences using InterProScan.
*The four json files can be browsed by importing them into https://www.ebi.ac.uk/interpro/result/InterProScan/#table.