High contiguity Arabidopsis thaliana genome with a single nanopore flow cell

We demonstrate a quick and cost effective reference assembly for an Arabidopsis thaliana accession using the USB-sized Oxford Nanopore MinION sequencer. We assemble the Arabidopsis accession KBS-Mac-74 into 62 contigs with an N50 length of 12.3 Mb covering 100% (119 Mb) of the non-repetitive genome. We demonstrate that the polished KBS-Mac-74 assembly is highly contiguous with BioNano optical genome maps, and of high base quality against a likewise polished Pacific Biosciences long-read assembly.本研究基于USB尺寸的牛津纳米孔MinION测序仪（Oxford Nanopore MinION），针对拟南芥（Arabidopsis thaliana）种质构建了一套快速且经济高效的参考基因组组装方案。本研究将拟南芥种质KBS-Mac-74组装为62个重叠群（contig），其N50长度达12.3 Mb，覆盖了该物种全部非重复基因组区域（总长度119 Mb）。经纠错优化后的KBS-Mac-74组装结果与BioNano光学基因组图谱（BioNano optical genome map）具有高度的连续性；同时，相较于同样经过纠错优化的太平洋生物科学（Pacific Biosciences）长读长组装结果，其碱基质量同样优异。