Gene expression changes in PC12 cells expressing GFP-mHtt-74Q treated with Guanabenz analogues

To gain an overview of the cellular impact of Guanabenz analogues, we analyzed the gene expression changes in PC12 cells expressing GFP-mHtt-74Q utilizing Affimatrix rat genome microarrays. Following exposure of PC12 cells expressing GFP-mHtt-74Q to Compound 6 for 48 hrs, we noted the enhanced expression of over 46 genes and the reduced expression of 8 genes compared to DMSO Genome-wide gene expression analyses (RTA-1_0, Affymetrix Rat Transcriptome Array 1.0, Affymetrix, Cleveland, OH, USA) were performed using RNA extracted from PC12 cells expressing GFP-mHtt-74Q, identified in four groups with 3 replicates for each group: non-induced and DMSO-treated, induced and DMSO-treated, induced and 10 µM Compound 3-treated and induced and 10 µM Compound 6-treated (at) for 48 hrs.