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Fecal microbiota in mice before and after repeated mild stress

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NIAID Data Ecosystem2026-05-02 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE222533
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To examine potential changes of the intestinal microbiota in mice caused by repeated mild stress, we profiled bacteria and fungi in the mouse feces by sequencing the 16s v3v4 region and the ITS1-2 region. Mice feces were collected before and after stress. Fecal microbial DNA were extracted by using QIAamp PowerFecal Pro DNA Kit (Cat. 51804). The 16s v3v4 regions and ITS1-2 regions were amplified by PCR with barcoded primers and sequenced with 2×300 paired-end reading. Data analysis were carried out with QIIME2(v2019.7)
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2024-12-26
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