Transcriptomic profiling of coaggregated Enterococcus faecalis and Fusobacterium nucleatum

To investigate the cell-cell interactions of intergeneric bacterial species, the study analyzed the transcriptomic profiling of coaggregated Enterococcus faecalis (Ef) and Fusobacterium nucleatum subsp. polymorphum (Fnp). Ef and Fnp were coaggregated in the coaggregation buffer. By using RNA-Seq analysis, gene regulation responses between mono-species and coaggregated species were compared, and differentially expressed genes were screened using DESeq2. Real-time quantitative polymepolymerase chain reaction was carried out to verify the results of DEGs. Bioinformatics analysis concerning DEGs was performed through KEGG pathway, GO functional enrichment and protein protein interaction network.