Whole Blood Measurements from the SpaceX Inspiration4 Mission (RNA-seq, epitranscriptome, WGS, clonal hematopoiesis, CBC)

The SpaceX Inspiration4 mission was a 3-day mission with four private astronauts to low Earth orbit that occurred in September 2021. The crew collected biospecimen samples before, during, and after flight. One of these biospecimen collections included whole blood collected via venipuncture into PaxGene Blood RNA tubes (BD Biosciences, #762165), collected pre-flight (L-92, L-44, L-3) and post-flight (R+1, R+45, R+82, R+194). Total RNA was purified from each tube and mRNA was sequenced using the Oxford Nanopore Technologies direct RNA-sequencing kit on the PromethION. Data was used to generate differential gene expression profiles and annotate sites of m6A modification. Additionally, whole genome sequencing was performed on crew members at L-44 and R+45 and a clonal hematopoiesis panel was performed on a subset of 9 genes (DNMT3A, TET2, ASXL1, TP53, CHEK2, JAK2, SRSF2, SF3B1, PPM1D). For these, DNA was isolated from the pellet of a cfDNA blood collection tube (Streck, cat #230470) (L-44, L-3, R+1, R+45, R+82, R+194). Finally, whole blood collected in K2 EDTA tubes (BD Biosciences, cat# 367844) were submitted to Quest Diagnostics for a complete blood count at all ground timepoints (L-92, L-44, L-3, R+1, R+45, R+82, R+194).

搜集汇总

背景与挑战

背景概述

该数据集来自SpaceX Inspiration4任务，收集了四名私人宇航员在为期三天的太空飞行前后多个时间点的全血样本，用于多组学分析，包括RNA-seq、表观转录组测序、全基因组测序、克隆造血检测和全血细胞计数。数据集特点在于整合了多种先进测序技术（如Oxford Nanopore、Element Biosciences、Illumina），旨在研究太空环境对基因表达、RNA修饰和血液系统的动态影响，为太空医学提供关键分子数据。