Ip, Wallace (2010) CIL:2879, Oryctolagus cuniculus, skeletal muscle cell. CIL. Dataset

Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in 0.2% saponin. The fibers were quick-frozen by contact with a liquid helium-cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

Images were recorded on Kodak 4489 film at 75,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Stereo pair with CIL:2880.

兔psoas骨骼肌纤维在0.2%皂素溶液中保持恒定长度，并通过浸泡引发僵硬。肌纤维通过与液氦冷却的铜块接触进行快速冷冻，并在Heuser型冷冻压机中进行冷冻断裂。随后，样品以Pt-C在24度角进行旋转复制，并在操作于100 kev的JEOL 100CX2透射电子显微镜下进行可视化。图像以75,000倍放大率记录在柯达4489胶片上。在+5度和-5度处拍摄立体对。胶片使用尼康Coolscan 9000ED扫描仪进行扫描，像素间距为20微米。与CIL:2880关联的立体对。