Transcriptome analysis of oocytes and early embryos to demonstrate trancriptional regulation during mouse oocyte-to-embryo transition

In this study, we performed RNA-seq to elucidate how transcriptional activities of mouse oocytes and preimplantation embryos are regulated to control oocyte-to-embryo transition. Through analyzing transcriptome alterations of transgenically-modified growing oocytes, early embryos from aged female, early embryos treated with drugs disturbing proteostasis, and embryonic stem cells treated with drugs disturbing proteostasis, we identified critical roles of proteostasis in transcriptional regulation of mouse oocytes and early embryos. To elucidate transcriptome changes of mouse oocytes and early embryos, we collected genetically-modified growing oocytes from P14 ovaries, early embryos cultured in KSOM with indicated drugs, and early embryos from aged female mice, and performed low-Input RNA Sequencing. For transcriptome analysis of mouse embryonic stem cell AB2.2, we extracted total RNA, followed by polyA RNA capture for standard RNA-seq library preparation.