Gene expression and alllele inheritence in xiphophorus spontaneous melanoma in backcross BC1 interspecies animals in Xiphophorus maculatus Skin. Gene expression and alllele inheritence in xiphophorus spontaneous melanoma in backcross BC1 interspecies animals in Xiphophorus maculatus Skin

RNA-Seq of spontaneous melanoma in Xiphophorus interspecies hybrids, and paired normal skin samples from the same melanoma-bearing animals were performed. Overall design: Gordan-Kosswig cross of X. maculatus-X. hellerii interspecies backcross were made. At age of 9 month, melanoma-bearing animals were identified and dissected. RNA from tumor/skin samples were isolated and sequenced for allelel inheritence analysis and overall gene expression profiling.