Spatiotemporal changes in Netrin/DSCAM1 signaling dictate axonal projection direction in Drosophila small ventral lateral clock neurons

Axon projection is a spatial and temporal-specific process in which the growth cone receives environmental signals guiding axons to their final destination. However, the mechanisms underlying changes in axonal projection direction without well-defined landmarks remain elusive. Here, we present evidence showcasing the dynamic nature of axonal projections in Drosophila's small ventral lateral clock neurons (s-LNvs). Our findings reveal that these axons undergo an initial vertical projection in the early larval stage, followed by a subsequent transition to a horizontal projection in the early-to-mid third instar larvae. The vertical projection of s-LNv axons correlates with mushroom body calyx expansion, while the s-LNv-expressed Down syndrome cell adhesion molecule (Dscam1) interacts with Netrins to regulate the horizontal projection. During a specific temporal window, locally newborn dorsal clock neurons (DNs) secrete Netrins, facilitating the transition of axonal projection direction in..., , , # Data from: Spatiotemporal changes in Netrin/DSCAM1 signaling dictate axonal projection direction in Drosophila small ventral lateral clock neurons

This dataset contains experimental data from a study aimed at investigating the development process of axon projection in s-LNvs. The data includes measurements of vertical axon projection distances and horizontal projection A.U. (arbitrary units) under different experimental conditions. The results of this study are discussed in detail in our relevant publication.

#### **Description of the data and file structure**

The xlsx data supports Figures 1, 2, 3, 4, 5, 6, Figure 2-Figure supplement 1, Figure 3-figure supplement 1, Figure 3-figure supplement 2, Figure 4-figure supplement 1 Figure 5-figure supplement 1, Figure 6-figure supplement 1.

**Figure1-source data 1 Related to figure1B**:  data underlying in the main text. This file contains average vertical projection length at different developmental stages. Figure 1B: Average vertical p...