Random genomic sequencing and analysis of strain CBS 94 by the Genolevures Consortium as part of a comparative genomics study of hemiascomycete yeasts. Candida tropicalis CBS 94

In a large-scale comparative genomics analysis of hemiascomycetes yeasts, the Genolevures Consortium partially sequenced the genomes of 13 yeasts, including that of Candida tropicalis strain CBS 94. For each species, they prepared a random genomic DNA library containing 3- to 5-kb inserts and performed single-pass sequencing (up to 1 kb) of both ends of the inserts, resulting in random sequence tags (RSTs). The Genolevures Consortium generated 2,541 RSTs, totaling 2.3 Mb and representing approximately 0.2X genome coverage, for Candida tropicalis. They assembled these sequences into contigs and annotated them based on sequential comparisons to DNA and protein sequences from Saccharomyces cerevisiae, protein sequences from other completely sequenced organisms, and protein sequences from all other organisms. The Candida tropicalis sequences are available in GenBank/EMBL/DDBJ under the accessions AL438875 to AL441602.