LC-MSMS analysis of HFF1 cells exposed to bear and bovine serum

The serum of hibernating bears has been shown to exhibit cytoprotective, anticatabolic effects when applied to human or mouse skeletal muscle cells. It is also able to reduce mitochondrial respiration in cells obtained from bear adipose tissue. Its action notably might involve reprogramming of transcriptional profiles, as shown in brown bear adipocytes and more recently in human muscle cells. However, the effects of bear serum on human cells has not been evaluated yet at the proteome level. The main objective of this study was to fill this gap by comparing the effects of hibernating bear serum with those of non-hibernating bear serum and foetal bovine serum on human fibroblasts.