Evaluation of the structure and storage stability of emulsions stabilized with native and modified diluted alkali-soluble pectin (DASP)

This study investigated the structure–function relationship of native and enzymatically modified diluted alkali-soluble pectin (DASP) and its impact on the stability of oil-in-water emulsions during storage. The work focused on understanding how enzymatic modification of RG-I-rich pectin fractions influences molecular architecture, rheological behaviour, interfacial properties, and emulsion stability under different pH conditions. Native and modified DASP fractions were characterized using compositional analysis, spectroscopic techniques, rheological measurements, and emulsion stability assessment combining macroscopic observations and microscopy. The dataset contains raw experimental data supporting physicochemical characterization of DASP fractions and evaluation of emulsion stability, including creaming behaviour, rheology, FTIR spectroscopy, and structural analysis. The folder entitled “Creaming index (%)” contains raw data files (.xlsx) used to calculate creaming index values during storage experiments. The datasets include direct measurements performed using a caliper. The folder entitled “DM (%) DA (%)” contains raw analytical data (.xlsx) used for determination of degree of methylation (DM) and degree of acetylation (DA). The files include calibration curves for methanol and acetic acid, as well as integrated peak areas corresponding to analysed samples. The folder entitled “FTIR” contains raw Fourier-transform infrared spectroscopy datasets, including unprocessed spectra (wavelength and absorbance values) collected in experimental replicates for both native and enzymatically modified DASP samples. The folder entitled “Microscopic pictures” contains a set of original microscopy images (.JPG) acquired for emulsions on each day of the storage test. The images are organised according to pH conditions. The folder entitled “Monosaccharide composition, UA content” contains Microsoft Excel files (.xlsx) presenting raw compositional data, including quantified values of individual monosaccharides and uronic acids obtained in experimental replicates. The folder entitled “Rheology” contains raw rheological datasets exported directly from the rheometer, including original instrument export files (.txt) and Microsoft Excel files (.xlsx). The datasets include viscosity measurements of emulsions collected during a 28-day storage study, separated according to pH conditions (pH 2.5 and pH 6.0). In addition, the folder contains flow curve data for pectin solutions as well as viscosity measurements for both pectin solutions and corresponding oil-in-water emulsions. These files provide complete raw datasets used for rheological analysis.本研究探究了天然及酶改性稀碱溶性果胶（diluted alkali-soluble pectin, DASP）的结构-功能关系，及其对水包油乳液储存过程稳定性的影响。本工作聚焦于解析富含鼠李糖半乳糖醛酸聚糖I（Rhamnogalacturonan I, RG-I）的果胶组分经酶改性后，在不同pH条件下对分子结构、流变行为、界面特性及乳液稳定性的调控机制。研究通过组分分析、光谱技术、流变测量以及结合宏观观测与显微成像的乳液稳定性评估方案，对天然与改性DASP组分完成了系统表征。 本数据集包含支撑DASP组分理化表征与乳液稳定性评价的全部原始实验数据，涵盖乳析行为、流变学、傅里叶变换红外光谱（Fourier-transform infrared spectroscopy, FTIR）及结构分析相关内容。 名为"Creaming index (%)"的文件夹包含用于计算储存实验中乳析指数值的原始数据文件（.xlsx），数据集包含使用游标卡尺获取的直接测量数据。 名为"DM (%) DA (%)"的文件夹包含用于测定甲基化程度（degree of methylation, DM）与乙酰化程度（degree of acetylation, DA）的原始分析数据（.xlsx），文件包含甲醇与乙酸的校准曲线，以及对应分析样品的积分峰面积。 名为"FTIR"的文件夹包含原始傅里叶变换红外光谱数据集，涵盖天然与酶改性DASP样品多组重复实验采集的未处理光谱数据（波长与吸光度值）。 名为"Microscopic pictures"的文件夹包含储存测试每日获取的乳液原始显微图像（.JPG），图像按pH条件进行分类整理。 名为"Monosaccharide composition, UA content"的文件夹包含展示原始组分数据的Microsoft Excel文件（.xlsx），数据涵盖实验重复获取的各单糖与糖醛酸定量值。 名为"Rheology"的文件夹包含直接从流变仪导出的原始流变学数据集，包含原始仪器导出文件（.txt）与Microsoft Excel文件（.xlsx）。数据集包含28天储存研究中采集的乳液粘度测量数据，按pH条件（pH 2.5与pH 6.0）进行分类。此外，该文件夹还包含果胶溶液的流动曲线数据，以及果胶溶液与对应水包油乳液的粘度测量数据，所有文件均为流变分析所用的完整原始数据集。