Sulforaphene attenuates multinucleation of pre-osteoclasts by suppressing expression of cell–cell fusion-associated genes <i>DC</i>-<i>STAMP</i>, <i>OC</i>-<i>STAMP,</i> and <i>Atp6v0d2</i>

We assessed the effect of sulforaphene (SFE) on osteoclast differentiation. SFE significantly decreased the number of RANKL-induced tartrate-resistant acid phosphatase-positive cells and suppressed pre-osteoclast multinucleation. Furthermore, SFE downregulated mRNA expression of <i>DC</i>-<i>STAMP</i>, <i>OC</i>-<i>STAMP,</i> and <i>Atp6v0d2</i>, which encode cell–cell fusion molecules. Our data suggest that SFE attenuates pre-osteoclast multinucleation via suppression of cell–cell fusion.

本研究评估了萝卜硫烯（sulforaphene, SFE）对破骨细胞分化的影响。实验结果表明，SFE可显著减少核因子κB受体活化因子配体（Receptor Activator for Nuclear Factor κB Ligand, RANKL）诱导的抗酒石酸酸性磷酸酶阳性细胞数目，并抑制破骨前体细胞的多核化过程。此外，SFE下调了编码细胞间融合分子的*DC-STAMP*、*OC-STAMP*及*Atp6v0d2*的mRNA表达水平。本研究数据提示，SFE可通过抑制细胞间融合，减弱破骨前体细胞的多核化进程。