RNA-seq on Fixed and Permeabilized Plasma cells

Here we developed a protocol to extract total RNA from fixed and permeabilized cells that were intracellularly stained and FACS isolated. We applied these techniques to derive the transcriptome of influenza- and BCR isotype-specific antibody secreting cells or plasma cells. These data reveal that ASC isotypes have more distinct transcriptional signatures than previously appreciated. Overall design: RNAseq on fixed, permeabilized, and sorted antibody secreting cells