Restoration of systemic growth by dNf1 and cAMP/PKA involves different tissues.

Act5C-Gal4 driven ubiquitous dNf1 re-expression, or elav-Gal4 and Ras2-Gal4 driven neuronal re-expression rescues the dNf1 pupal size defect, whereas dnc RNAi or UAS-PKA* expression controlled by the same drivers is ineffective. By contrast, expressing dNf1 in specific parts of the neuroendocrine ring gland with the Akh-Gal4, Feb36-Gal4 or Aug21-Gal4 drivers fails to rescue, whereas using the same drivers to express dnc RNAi or attenuated UAS-PKA* transgenes does increase dNf1 pupal size. All crosses produced viable adults unless otherwise indicated.†denotes lethality, SV sub-viable, n/a not applicable, NR non-rescue.The data shown summarize results of a larger effort to identify the tissues in which dNf1 and cAMP/PKA affect systemic growth. Full results are shown in Table S5.