Multi-omic and spatial analysis of mouse kidneys highlights sex-specific differences in gene regulation across the lifespan

We utilized the Xenium single-cell platform to analyze spatial expression patterns for kidneys from female and male mice at W12 and W92. Four distinct kidney zones were identified: the cortex, the outer stripe of the outer medulla (OSOM), the inner stripe of the outer medulla (ISOM), and the inner medulla (IM) based on their histology and cell type compositions. Female kidneys had a thinner cortex and thicker OSOM, while males had a vastly larger IM, contributing to sex-based size differences. We utilized the Xenium single-cell platform to analyze spatial expression patterns for kidneys from female and male mice at W12 and W92.