Generational sperm DMR.

DMR numbers for F1, F2, F3 generation sperm. DMRs were defined using an edgeR p-value of 10−5, 10−6 and 10−9 for the F1, F2 and F3 generations, respectively. The DMR edges were extended to include neighboring windows with a p-value less than 0.1. Genomic windows are considered neighboring if they are within 1000 bp of the DMR boundary. In other words, DMR edges are extended until there is no genomic window within 1000 bp with a p-value less than 0.1. The number of DMRs found using different p-value cutoff thresholds is presented. The All Window column shows all DMRs. The Multiple Window column shows the number of DMRs containing at least two significant windows. (A) F1 generation sperm DMR numbers at various p value thresholds with p<10−6 selected for further analysis. (B) F2 generation sperm DMR numbers at various p value thresholds with p<10−5 selected for further analysis. (C) F3 generation sperm DMR numbers at various p value thresholds with p<10−9 selected for further analysis.