Assessing the impact of tooth extraction on cognitive function through single-cell sequencing of the mouse hippocampus

Recent studies have indicated that oral health significantly influence brain functions, particularly cognitive abilities. Periodontal diseases and tooth loss may contribute to brain function impairment. This study demonstrates through a series of behavioral and molecular biology experiments that galanin likely plays a crucial role in mediating the impact of tooth extraction injury on cognitive function. To further elucidate the underlying mechanisms, hippocampal tissues were collected from mice of control group, molar-extracted group (ME), and group treated with M35 (a galanin inhibitor) after tooth extraction for single-cell RNA sequencing analysis, aiming to evaluate the effects of tooth extraction injury signals on the hippocampal microenvironment.