In vitro differentiation of mouse liver ductal organoids

Liver ductal organoids are liver tissue stem cell-derived organoids which has bipotential stemness capacity to hepatocyte and cholangiocyte. However, conventional hepatocyte differentiation methods are insufficient, resulting in hepatocyte-like cells with low hepatocyte marker expression retaining high cholangiocyte marker expression. The goal of this study is to find a new hepatocyte differentiation method by using transduction of hepatocyte specific transcription factors. The data shows the RNA-seq data of liver ductal organoids which were induced to hepatocyte differentiation by the conventioonal methods (DM-GFP) and our method, i.e. four transcription factor transuction (DM-4TF), comparing with liver ductal organoids cultured in stem ness maintenance medium (EM) and mouse primary hepatocyte cultured for 48 hours (MPH).