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Long-term research revealed that the application of biochar significantly increased CH₄ emissions by 38% while reduced N₂O emissions by 29%, and greatly increased soil carbon storage by 87173%, significantly while decreasing CF by 1.1.8 Mg CO₂ eq ha^-1. Among them, CH₄ and N₂O emissions contributed 4695% of total GHG emissions, and the production and transportation of nitrogen fertilizer and biochar contributed 152% of total GHG emissions. Nitrogen fertilizer application significantly increased rice yield by 85% and resulted in the highest NEEB. On the contrary, the application of biochar had a slight effect on rice yield and brought negative NEEB. However, the combination of biochar and nitrogen fertilizer can significantly increase rice yield while reducing CF, and bring positive NEEB.