Gene expression profiles of WT and epithlial GSDMD ko mouse intestine [scRNA-seq]

The small intestine (SI) is the first site of absorption for life-sustaining nutrients and a gateway for external pathogens and toxins. Diet enables the host to initiate the SI absorptive machinery, while maintaining the barrier activity and a strict immune homeostasis. To uncover the role of epithelial GSDMD in SI physiology, we conditionally deleted Gsdmd in intestinal epithelial cells by generating Villin-cre+; Gsdmdf/f (GsdmdIEC) mice小肠（Small Intestine，SI）是维持生命的营养物质的首要吸收部位，同时也是外界病原体与毒素侵入的门户。饮食可诱导宿主启动小肠的吸收功能机制，同时维持肠道屏障活性与严格的免疫稳态。为揭示上皮细胞中GSDMD在小肠生理过程中的作用，我们通过构建Villin-cre+; Gsdmdf/f（GsdmdIEC）小鼠，在肠上皮细胞中条件性敲除了Gsdmd基因。