Natural killer cells suppress T cell tumor immune evasion [B16-F10 CRISPR/Cas9 MHC I low]

Investigation of tumor genes which are involved in the upregulation of MHC I expression through a genome-wide in vitro CRISPR/Cas9 screen Overall design: B16-F10 Cas9 expressing cells transduced with a genome-wide CRISPR library were successively treated overnight with IFN-? and FACS sorted for cells that failed to upregulate MHC I expression. An untreated population of transduced cells frozen after puromycin selection were used for comparison