Spatial Transcriptomic Profiling of the Mouse Adrenal Gland and Adjacent Adipose Tissue

This study employs spatial transcriptomics to map the molecular landscape of the mouse adrenal gland, focusing on distinct cortical zones (zona glomerulosa, zona fasciculata, X-zone) and the adrenal medulla, as well as adjacent adipose tissue. Using Visium CytAssist technology, we analyzed adrenal sections from male CD-1 IGS mice, identifying seven distinct cell clusters, including brown and white adipose tissues. Four sexually mature male CD-1 IGS mice, 10 weeks old with a body weight of approximately 37 g were used in this study. Mice were housed in colony cages in a pathogen-free environment with temperature maintained at 21-23C and relative humidity between 55-60% and under a 12h light/12 h dark cycle. All mice were given standard chow and water ad libitum. The mice were euthanized by decapitation and the harvested adrenals were immediately preserved in 10% buffered formalin for further analysis.