High-resolution genome topology analysis of human peripheral blood mononuclear cells and osteoclasts.

Genome topology analysis of human osteoclasts (Micro-C). In this study we performed Micro-C analysis (Dovetail Genomics) on peripheral blood mononuclear cells and osteoclasts (OC) cultured in vitro, derived from blood samples of 4 healthy human participants. This experiment provided genome topology and chromatin conformation data on these cells to facilitate efficient targeting of ongoing functional genomics studies. We generated a unique high-resolution genome topology dataset for human OC, detecting chromatin loops across the genome. This data should be relevant to studies of the genetics of osteoporosis and to inform future functional studies of osteoclasts.

Published as: Wilson SG, Campbell PJ, Sooraj D, Leatherbarrow K, Mullin BH, Brown SJ, Zhu K, Mullin S, Ward BK, Zhang J, Torchia J, Dudbridge F, Xu J, Pavlos NJ, Chandler D, Walsh JP. Genome topology analysis and transcriptomics of human osteoclasts reveals enhancer-promoter interactions at loci for bone traits and diseases. JBMR Plus. 2025 Jul 16;9(10):ziaf120. doi: 10.1093/jbmrpl/ziaf120. PMID: 40918604; PMCID: PMC12409414.