Single-cell multiomic analysis identifies macrophage subpopulations in promoting cardiac repair

Our study comprehensively investigated the epigenetic features of cardiac F4/80+CD45+ macrophages at 3 and 7 days after P1 and P10 MI at infarct, border, and remote zones of mouse hearts (IZ, BZ, and RZ) through single cell H3K27ac ChIP-seq. we identified 9 subclusters and analyzed their potential functions, cluster-specific enhancer features, and enrichment of cell-type specific transcription factors. Surprisingly, we found intracluster epigenetic heterogeneity exists with biological functions. Moreover, we identified two MI-responsive lineages and targeted one inflammatory lineage exhibiting obvious pre-regenerative function. Overall design: Mouse hearts were collected at 3 days and 7 days after P1 and P10 MI. The heart tissues were roughly divided into three zones and then digested with collagenase type II enzyme at 37°C for 30 min to generate single-cell suspension. F4/80/CD45+ cardiac macrophages were collected by FACS sorting and then subjected to single cell H3K27ac CoBATCH profiling. Please note that each bw file was genearated from both replicates and is linked to the corresponding *rep1 sample records.