Figure S1 - Inflammation-Induced Reactivation of the Ranavirus Frog Virus 3 in Asymptomatic Xenopus laevis

Detection of infectious virus in kidneys but not PLs of reactivated asymptomatic infection. PCR assay on total DNA purified from PBLs and erythrocytes from blood collected from 15 different frogs at 30 dpi, and from the same animals 3 days after bacterial stimulation (35 dpi). Presence of viral DNA was assed by PCR on 50 ng of total DNA using FV3 specific primers for MCP and vDNA poly II as well as X. laevis Ef-1α as a loading control. (PDF)