Transcriptomic characterization of the human cell cycle in individual unsynchronized cells. Transcriptomic characterization of the human cell cycle in individual unsynchronized cells

Here, we profiled transcription across the cell cycle in single human myxoid liposarcoma cells, without prior synchronization. Flourescence-activated cell sorting was used to infer G1, S or G2/M cell cycle stages after staining cells for DNA content. Single-cell RNA-sequencing was performed according to the Smart-seq2 protocol.