Purification and characterization of the diphtheria toxin repressor.
收藏PubMed Central1992-08-15 更新2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC49753/
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资源简介：
The diphtheria toxin repressor gene (dtxR) encodes a protein (DtxR) that regulates transcription of the diphtheria toxin gene (tox) by an iron-dependent mechanism. Cloned dtxR was expressed in Escherichia coli from the phage T7 gene 10 promoter, and DtxR was purified. Specific binding of DtxR to the tox+ operator was dependent on reduction of DtxR and the presence of ferrous ions. DtxR protected a sequence of approximately 30 nucleotide pairs, partially overlapping the tox promoter and containing a region of dyad symmetry, from digestion by DNase I. DtxR exhibited very little binding to the mutant tox-201 operator region and failed to bind to the promoter/operator region of the ferric uptake regulation (fur) gene of E. coli. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1992-08-15
