Expression data of human melanoma cells plus/minus Androgen Receptor (AR) silencing by two independent shRNAs

Cancer susceptibility of male versus female individuals is significantly different in melanoma, with females showing greater survival than males. Accrued information on the role fo sex hormones in melanoma is mostly limited to estrogen signaling, with only circumstantial evidence related to androgen receptor. We used Clariom™ D GeneChip Assay to study the global gene expression changes upon silencing of AR by two independent shRNAs in three human melanoma cell lines. Three different human melanoma cells were infected with lentivirus containing either two independent shRNAs against AR or control shRNA. 48 hours after virus transfection, cells were selected for puromycin resistance for 3 days before RNA extraction.