Sequence analysis of the metagenomic inserts isolated in this study: taxonomical and functional assignation, and gene occurrence in the human gut metagenome.

aContig 7 is partially redundant with contig 8 (ORFs 1 to 14 of contig 7 are identical to ORFs 5 to 19 of contig 8), contig 11 is partially redundant with contig 14 (ORFs 9 to 12 and 18 to 24 of contig 11 are identical to ORFs 11 to 22 of contig 14). In addition, contig sequences of clones 15, 16 and 17 were totally covered by contig 1, 2 and 3 sequences, respectively.bMEGAN based assignation.cCAZy families known to degrade the glycosidic bonds contained in the prebiotic substrate.dResults of BLASTN comparison of the predicted ORFs with the metagenomic data sets issued from fecal sampling of 3 cohorts of 163, 139 and 110 subjects. The first number corresponds to the number of ORFs that present a sequence identity ≥90% and an E-value = 0 with a gene detected at least in one subject of the three cohort. The second number corresponds to the number of ORFs that present a sequence identity ≥90% and an E-value = 0 with a gene detected at least in 20 subjects.