Effect of Ptpn2 KO and IFNγ on axon regeneration

To investigate the function of Ptpn2, IFNγ and cGAS on axon regeneration, we performed gene transcription profiling of dorsal root ganglion neurons from 5 groups (WT, cKO, WT injured, cKO injured, RU521 injured) and retinal ganglion cells from 4 groups (WT, IFNγ, cKO, IFNγ+cKO). For DRG experiment, cGAS inhibitor RU521 was injected to the sciatic nerve of wildtype mice. Nerve samples were collected 3 days after injury and RNA was extracted for sequencing. For RGC experiment, AAV-Cre or GFP was injected to the eyes of Ptpn2 flox mice 2 weeks before optic nerve injury. IFNγ was injected to the eyes right after injury. Retinal ganglion cells (WT,IFNγ,cKO,cKO+IFNγ) were collected 3 days after injury and RNA was extracted for sequencing.